Contamination and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood in a place of Zhejiang Province / 上海预防医学
Shanghai Journal of Preventive Medicine
;
(12): 500-502, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-881495
ABSTRACT
Objective:
To understand the contamination and antimicrobial resistance of Vibrio parahaemolyticus in seafood sold in a place of Zhejiang Province, and to provide basic data for the prevention and control of food-borne diseases caused by V. parahaemolyticus.Methods:
V. parahaemolyticus was isolated and identified from seafood according to GB 4789.7—2013 method. The virulence genes were identified by real-time fluorescent PCR, and the antimicrobial sensitivity test was performed by Kirby-Bauer method.Results:
V. parahaemolyticus was isolated from 55 of 210 seafood samples. The detection rate was 26.19%, and the detection rates of different species were quite different. None of the 55 isolates contained virulence genes tdh and trh, while all isolates have species-specific genes tlh and toxR. Most isolates were resistant to ampicillin and amoxicillin, with the same antimicrobial resistance rate of 96.36%. All isolates were fully sensitive to ampicillin/sulbactam, tetracycline, ciprofloxacin, levofloxacin, loxacin and chloramphenicol.Conclusion:
V. parahaemolyticus contamination is common in sold seafood. The antimicrobial resistance of the isolates has reached a certain level. Monitoring of the pollution status of V. parahaemolyticus in seafood should be strengthened and the aquaculture and clinical use of antibiotics should be standardized.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Shanghai Journal of Preventive Medicine
Year:
2021
Type:
Article
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