Effects of maltol aluminum exposure on hippocampus neuroligin 1 and long-term potentiation in rats / 中国职业医学

ABSTRACT

OBJECTIVE: To explore the effects of sub-chronic aluminum exposure on the combination of hippocampus neuroligin 1( NL1) with N-methyl-D-aspartate receptor( NMDAR) and the effects of correlated bonding to long-term potentiation( LTP) in rats. METHODS: Ninety healthy male specific pathogen free SD rats were selected and randomly divided into blank control group,solvent control group and low-,medium-and high-dose groups,with 18 rats in each group. The rats in blank control group received no treatment. Rats in solvent control group were given 0. 9% sodium chloride solution by concentration of 1 m L/kg body weight( bw). The low-,medium-and high-dose group rats were given the mass concentration of 0. 41,0. 81,1. 62 mg/kg bw maltol aluminum solution by intraperitoneal injection every other day for 1,2,and 3 months,respectively. After maltol aluminum exposure,LTP was detected in the CA1 region of rat hippocampus,aluminum levels were detected by the graphite furnace atomic absorption spectrometry,and the protein relative expression of NL1 combined with NMDAR1 and NMDAR2B were detected by immunoprecipitation and immunoblotting. RESULTS: The LTP in the solvent control group and the low-,medium-,high-dose groups were all lower than that in the blank control group( P < 0. 01). The LTP in the high-dose group was lower than those in the solvent control group and the low-and medium-dose groups( P < 0. 05). The aluminum levels in the hippocampus of rats in the low-,medium-and high-dose groups were higher than those of the blank control group and the solvent control group( P <0. 01). The protein relative expression of NMDAR1 and NMDAR2B combined with NL1 in treatment groups was lower than those in the blank control group and solvent control group at the same exposure time points( P < 0. 01). The protein relative expression of NMDAR1 and NMDAR2B combined with NL1 in treatment groups at time point of 2 months was lower than those at time point of 1 month in the same dose group( P < 0. 01). The protein relative expression of NMDAR1 and NMDAR2 B combined with NL1 at time point of 3 months was lower than those at time points of 1 and 2 months in the same dose group( P < 0. 01). CONCLUSION: Maltol aluminum can prevent the normal combination of NL1 with NMDAR1 and NMDAR2B,affecting LTP regulated by NMDAR1 and NMDAR2B,resulting in a LTP decline,as well as learning and memory impairment.