Detecting thiocyanate in human urine by precolumn derivatization-high performance liquid chromatography / 中国职业医学

ABSTRACT

OBJECTIVE: To establish a method for detecting thiocyanate in human urine by high performance liquid chromatography( HPLC) with 2,3,4,5,6-pentafluorobenzyl bromide as precolumn derivatization reagent.METHODS: Thiocyanate in human urine was derived with 2,3,4,5,6-pentafluorobenzyl bromide, and separated by poroshell 120EC-C18 column with acetonitrile:deionized water( 60:40,V/V) as mobile phase.detected by HPLC,Liquid chromatography-UV detector was used for determination.The wavelength was 212.00 nm.RESULTS: Good linearity was obtained in the range of 0.05-10.32 mg/L with the correlation coefficient of 0.999.The detection limit was 6.31 μg/L and the minimum detection concentration was 63.10 μg/L( 0.1 mL urine).The recovery rate was 95.1%-102.9%.The within-run relative standard deviation( RSD) and the between-run RSD were 0.9%-1.0% and 0.9%-2.1%,respectively.The urine samples could be stored at 4 ℃ for 7 days.CONCLUSION: This method has high sensitivity,good specificity and sample preparation,which can be used for detecting urine thiocyanate in occupational population.