Determination of 2,5-hexanedion in urine by liquid-liquid extraction-gas chromatography / 中国职业医学

ABSTRACT

OBJECTIVE: To establish a method for rapid determination of 2,5-hexanedion in urine by liquid-liquid extraction-gas chromatography. METHODS: Two mL of urine simple was acidified using 100 μL of hydrochloric acid. Anhydrous sodium sulfate 0.5 g was added for salting out. Extraction was carried out using 1 mL of chromatographic grade ethyl acetate vortex, separated by Rtx®-WAX chromatographic column, and detected with flame ionization detector. RESULTS: The good linear range of 2,5-hexanedion was 0.25-15.00 mg/L. The correlation coefficient was 0.999 7. The detection limit was 0.08 mg/L and the minimum detection concentration was 0.25 mg/L. The average recovery rate was 93.0%-110.0%. The within-run relative standard deviation(RSD) was 1.9%-5.4% and the between-run RSD was 5.0%-11.2%. CONCLUSION: This method is simple, sensitive and accurate, which is suitable for detecting urine 2,5-hexanedion in occupational n-hexane exposed workers and suspected patients with occupational chronic n-hexane poisoning.