Determination of 16 PAHs in workplace air by high performance liquid chromatography with ultraviolet/fluorescence detection / 中国职业医学

ABSTRACT

OBJECTIVE: To establish a method for simultaneous detection of 16 kinds of gas and particle-phase polycyclic aromatic hydrocarbons(PAHs) in workplace air by high performance liquid chromatography with ultraviolet and fluorescence detector(HPLC-UV-FLD).METHODS: PAHs in workplace air were collected by polytetrafluoroethlene filter with XAD-2 tube. The samples were added with 5 mL of acetonitrile. The samples were extracted by ultrasound and eluted with acetonitrile-water gradient. Ultraviolet with tandem fluorescence detector was used for determination.RESULTS: Eleven kinds of PAHs showed a good linear range of 1.0-400.0 μg/L. Five kinds of PAHs showed a good linear range of 10.0-2 000.0 μg/L and the linear correlation coefficient was greater than 0.999 0. The minimum detection concentration was 0.000 01-0.000 67 mg/m~3 (75 L air sample). The average elution efficiency of filter on granular PAHs except for naphthalene and acenaphthene ranged from 71.0% to 99.0%. The average desorption efficiencies of adsorbent tube was 43.0%-90.0%. The within-run relative standard deviations(RSD) was 0.3%-8.3%, and the between-run RSD was 0.0%-9.2%.CONCLUSION: This method is simple, sensitive, has good linear range and precise. It is suitable for simultaneous detection of PAHs in workplace air.