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Toxic effect of nano-cerium oxide on zebrafish embryonic early development / 中国职业医学
China Occupational Medicine ; (6): 48-52, 2020.
Article in Chinese | WPRIM | ID: wpr-881863
ABSTRACT

OBJECTIVE:

To study the effect of nano-cerium oxide on the early development of zebrafish embryos.

METHODS:

The well-developed zebrafish embryos were randomly divided into the control group and the 50, 100, 200, 400 and 800 mg/L dose groups, with 40 embryos in each group. The dose groups were treated with nano-cerium oxide at the corresponding mass concentration for 5 days. The control group received no treatment. The death and malformation of embryos were observed. The heart rate of zebrafish embryos was recorded using confocol microscope. The protein expression of microtubule-associated protein 1 light chain 3(LC3) and cleaved Caspase-3 and were observed by Western blot technology.

RESULTS:

The death and embryonic malformation rate of zebrafish embryos increased with the increase of doses, showing statistical significance(P<0.01). The heart rate of the 800 mg/L dose group was decreased compared with the control group [(77±8) vs(93±4) beats/min, P<0.01]. There was no statistical significant difference in LC3-Ⅱ protein expression in each groups(P>0.05). The cleaved Caspase-3 protein expression increased in all dose groups compared with the control group(P<0.05). The cleaved Caspase-3 protein expression in the 200 mg/L dose group was higher than that in the 50 mg/L dose group(P<0.05).

CONCLUSION:

The nano-cerium oxide may induce cell apoptosis, causing toxic effect in early development of zebrafish embryos.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Occupational Medicine Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Occupational Medicine Year: 2020 Type: Article