Determining 6 arsenic species in human urine by high performance liquid chromatography-atomic fluorescence spectrometry / 中国职业医学
China Occupational Medicine
;
(6): 204-208, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-881888
ABSTRACT
OBJECTIVE:
To establish a methodology using high performance liquid chromatography-atomic fluorescence spectrometry(HPLC-AFS) for the determination of 6 arsenic species including arsenite, dimethyl arsenic acid, monomethyl arsenic acid, dimethyl arsenic acid, monomethyl arsenic acid and arsenite in human urine.METHODS:
The PRP-X100 anion exchange chromatographic column was used with 4.0 mmol/L ammonium carbonate, ammonium sulfate and ammonium phosphate mixed solution with pH 8.9 as mobile phase. The carrier current was hydrochloric acid with volume fraction of 10.0% and reducing agent was potassium borohydride with mass fraction of 3.5%. After centrifugal separation of urine sample, 6 forms of arsenics were detected by HPLC-AFS.RESULTS:
The linear range of 6 forms of arsenics in urine was 3.90-100.00 μg/L. The correlation coefficient was 0.999 1-0.999 9, and the detection limit was 1.31-3.90 μg/L. The recovery rate was 91.2%-103.5%. The within-run and between-run relative standard deviations were 0.5%-4.9% and 1.8%-5.0%, respectively. The samples could be stored for at least 7 days under the temperature of-80 ℃.CONCLUSION:
The method has low detection limit, good precision and high sensitivity, which is suitable for the determination of arsenics in human urine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Occupational Medicine
Year:
2020
Type:
Article
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