Your browser doesn't support javascript.
loading
Determination of three components in Shenqi-Yiqi oral liquid with HPLC combined with switching wavelengthmethod / 国际中医中药杂志
International Journal of Traditional Chinese Medicine ; (6): 268-271, 2021.
Article in Chinese | WPRIM | ID: wpr-882582
ABSTRACT

Objective:

To establish the high performance liquid chromatography (HPLC) combined with switching wavelength method to determinate chlorogenic acid, ferulic acid, Calycosin-7-glucoside in Shenqi-Yiqi oral liquid.

Methods:

The Shenqi-Yiqi oral liquid was separated on an Agilent HC-C18 column by gradient elution using acetonitrile (A) - 0.1% phosphate (B) as the mobile phase. The flow rate was 1.0 ml/min. The detection wavelengths were 260 nm (chlorogenic acid and Calycosin-7-glucoside), 316 nm (ferulic acid) by wavelength conversion method.

Results:

The linear ranges of chlorogenic acid, ferulic acid, Calycosin-7-glucoside were 70.40-704.00 μg ( r=0.999 8), 1.36-13.60 μg ( r=0.999 8), 1.28-12.80 μg ( r=0.999 7), the average recovery rate were 99.44% ( RSD=2.54%), 101.06% ( RSD=1.57%), and 98.00% ( RSD=2.09%).

Conclusion:

This method is convient, rapid and accurate for determinating chlorogenic acid, ferulic acid and Calycosin-7-glucoside in Shenqi-Yiqi oral liquid.
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Journal of Traditional Chinese Medicine Year: 2021 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Journal of Traditional Chinese Medicine Year: 2021 Type: Article