Protective Effects of Immunization with Proteinase, Phospholipase and Cell Wall Proteins of Candida albicans in Mice Candidosis / 대한의진균학회지
Korean Journal of Medical Mycology
;
: 9-15, 2009.
Article
in Korean
| WPRIM
| ID: wpr-88366
ABSTRACT
BACKGROUND:
The opportunistic fungus Candida albicans is a major pathogen especially to immunocompromised patients.OBJECTIVES:
We examined the protective effect of the active and passive immunizations to evaluate the applicability for the treatment of candidosis in Candida-infected mice model.METHODS:
Candida cell wall components were obtained by treatment of lyticase, proteinase K, and dithiothreitol. The proteinase was purified from the culture filtrates of C. albicans using a series of chromatographic steps consisting of DEAE-Sepharose FF, Sephacryl S-200 HR and size-exclusion high performance liquid chromatography. The phospholipase was purified from the culture supernatant of C. albicans with DEAE column chromatography, reverse phase column chromatography, revere phase HPLC and size-exclusion HPLC. Antibodies to cell wall protein components, proteinase and phospholipase were produced by immunization into mice of same strain.RESULTS:
The mean survival times of active and passive immunized mice groups were longer than those of non-immunized groups.CONCLUSION:
These results showed that immunization with proteinase and its antibody were the most effective to prolong survival time in Candida-infected mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Hydrolases
/
Phospholipases
/
Acrylic Resins
/
Candida
/
Candida albicans
/
Proteins
/
Cell Wall
/
Survival Rate
/
Chromatography
/
Chromatography, High Pressure Liquid
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Medical Mycology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS