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miR-424-5p improves radiation sensitivity of cervical cancer by targeted inhibition of HMGA1 expression / 中华放射肿瘤学杂志
Article in Chinese | WPRIM | ID: wpr-884559
Responsible library: WPRO
ABSTRACT

Objective:

To investigate the effect of miR-424-5p on radiosensitivity and its mechanism in cervical cancer patients.

Methods:

The expression levels of miR-424-5p in the cervical cancer tissues and Hela cells were detected by RT-qPCR. The apoptosis rate of Hela cells was determined by flow cytometry. The proliferation activity of Hela cells was detected by CCK-8 assay. The protein expression levels in Hela cells were measured by Western blot.

Results:

Compared with normal tissues and cells, the expression level of miR-424-5p was significantly down-regulated in the cervical cancer tissues and Hela cells (1.03 vs. 0.88, P<0.01; 1.00 vs. 0.75, P<0.01). Overexpression of miR-424-5p significantly inhibited the proliferation activity of Hela cells after radiation treatment ( P<0.01), and significantly increased the apoptosis rate of Hela cells after radiation treatment (24.82% vs. 49.94%, P<0.001). Overexpression of miR-424-5p inhibited HMGA1 expression (1.01 vs. 0.63, P<0.01). miR-424-5p directly affected HMGA1, thereby impacting the radiosensitivity of cervical cancer radiotherapy.

Conclusion:

miR-424-5p can improve the radiosensitivity of cervical cancer radiotherapy by directly targeting HMGA1.
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Radiation Oncology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Radiation Oncology Year: 2021 Type: Article