Impact of Cell Density on Differentiation Efficiency of Rat Adipose-derived Stem Cells into Schwann-like Cells
International Journal of Stem Cells
;
: 213-220, 2016.
Article
in English
| WPRIM
| ID: wpr-88593
ABSTRACT
BACKGROUND AND OBJECTIVES:
Schwann-like (SC-like) cells induced from adipose-derived stem cells (ASCs) may be one of the ideal alternative cell sources for obtaining Schwann cells (SCs). They can be used for treating peripheral nerve injuries. Co-culture with SCs or exposure to glial growth factors are commonly used for differentiation of ASCs to SC-like cells. However, the effect of initial cell density as an inductive factor on the differentiation potential of ASCs into the SC-like cells has not been yet investigated. METHODS ANDRESULTS:
ASCs were harvested from rat and characterized. The cells were seeded into the culture flasks at three different initial cell densities i.e. 2×10³, 4×10³ and 8×10³ cells/cm² an overnight and differentiated toward SC-like cells using glial growth factors. After two weeks, the differentiation rate of ASCs to SC-like cells at different densities was assessed by immunofluorescence, fluorescence-activated cell sorting analysis and real time RT-PCR. Expression of the typical SCs markers, S-100 proteins and glial fibrillary acidic protein (GFAP) protein, was observed in all cell densities groups although the number of S100-positive and GFAP-positive cells, and the expression of p75(NTR) mRNA, another SC marker, were significantly higher at the density of 8×10³ cells/cm² when compared with the other cell densities groups (p<0.001).CONCLUSIONS:
The results suggest that the higher differentiation rate of ASCs to SC-like cells can be obtained at initial cell density of 8×10³ cells/cm², possibly via increased cell-cell interaction and cell density-dependent influence of glial growth factors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Schwann Cells
/
Stem Cells
/
RNA, Messenger
/
S100 Proteins
/
Cell Count
/
Fluorescent Antibody Technique
/
Coculture Techniques
/
Neuregulin-1
/
Peripheral Nerve Injuries
/
Flow Cytometry
Limits:
Animals
Language:
English
Journal:
International Journal of Stem Cells
Year:
2016
Type:
Article
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