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Optimization of a cucurbit6uril-based real-time label-free method for analyzing the activity of ornithine decarboxylase / 生物工程学报
Chinese Journal of Biotechnology ; (12): 2903-2914, 2021.
Article in Chinese | WPRIM | ID: wpr-887852
ABSTRACT
Ornithine decarboxylase (ODC) is a key enzyme in the biosynthetic pathway of polyamines and catalyzes the decarboxylation of ornithine to produce putrescine. Inhibition of ODC activity is a potential approach for the prevention and treatment of many diseases including cancer, as the expression levels and the activities of ODC in many abnormal cells and tumor cells are generally higher than those of normal cells. The discovery and evaluation of ODC inhibitors rely on the monitoring of the reaction processes catalyzed by ODC. There are several commonly used methods for analyzing the activity of ODC, such as measuring the yield of putrescine by high performance liquid chromatography, or quantifying the yield of isotope labelled carbon dioxide. However, the cumbersome operation and cost of these assays, as well as the difficulty to achieve high-throughput and real-time detection, hampered their applications. In this work, we optimized a real-time label-free method for analyzing the activity of ODC based on the macromolecule cucurbit[6]uril (CB6) and a fluorescent dye, DSMI (trans-4-[4-(dimethylamino) styryl]-1-methylpyridinium iodide). Finally, the optimized method was used to determine the activities of different ODC inhibitors with different inhibition mechanisms.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Ornithine / Ornithine Decarboxylase / Bridged-Ring Compounds / Putrescine / Ornithine Decarboxylase Inhibitors / Imidazoles Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Ornithine / Ornithine Decarboxylase / Bridged-Ring Compounds / Putrescine / Ornithine Decarboxylase Inhibitors / Imidazoles Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article