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Quality standard of Perillae Folium based on multicomponent determination with HPLC method / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-888062
Responsible library: WPRO
ABSTRACT
This research established the HPLC methods for the determination of perillaketone, perillaldehyde, caffeic acid, scutellarin, and rosmarinic acid in 33 batches of Perillae Folium. Kromasil C_(18)(4.6 × 250 mm, 5 μm) chromatographic column was used, and the mobile phase for determination of the perillaketone and perillaldehyde was methanol-water(55∶45) solution, at a flow rate of 1.0 mL·min~(-1), with the column temperature at 30 ℃. The mobile phase for the determination of caffeic acid, scutellarin and rosmarinic acid was methanol(A)-0.2% phosphoric acid aqueous solution(B) with gradient elution(0-20 min, 25%-30% A; 20-60 min, 30%-43% A). The flow rate was 1.0 mL·min~(-1) and the column temperature was set at 30 ℃. The results showed that the established method can achieve good separation of the five components in samples, with a good linear relationship and high accuracy, indicating that the methods can be used for the determination of Perillae Folium. The results showed that all samples contained five components. And the content of rosmarinic acid(0.04%-1.57%) > scutellarin(0.03%-0.77%) > perillaldehyde(0.02%-0.66%) > perillaketone(0.03%-0.30%) > caffeic acid(0.006%-0.07%). Thirty-three Batches of Perillae Folium can be grouped into 5 categories. There are certain content rules and region specificities under different clusters. Perillaketone, perillaldehyde, and rosmarinic acid can be used as the main markers to evaluate the quality of Perillae Folium.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Drugs, Chinese Herbal / Plant Extracts / Chromatography, High Pressure Liquid / Plant Leaves Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Drugs, Chinese Herbal / Plant Extracts / Chromatography, High Pressure Liquid / Plant Leaves Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2021 Type: Article