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Screening of interacting proteins of idiopathic gonadotropin-releasing hormone deficiency pathogenic gene RNF216 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics ; (6): 631-634, 2021.
Article in Chinese | WPRIM | ID: wpr-888362
ABSTRACT
OBJECTIVE@#To screen proteins interacting with ring finger protein 216(RNF216) through yeast two hybrid experiment, and further clarify the role of RNF216 in the pathogenesis of gonadotropin-releasing hormone deficiency.@*METHODS@#A recombinant expression vector pGBKT7-RNF216 was constructed and transformed into yeast Y2HGold, which was hybridized with a human cDNA library in order to screen proteins interacting with RNF216. The interaction was verified in yeast Y2HGold.@*RESULTS@#A recombinant expression vector pGBKT7-RNF216 was successfully constructed and expressed in yeast Y2HGold. Filamin B (FLNB) was identified by yeast two hybrid experiment, and their interaction was verified in yeast Y2HGold.@*CONCLUSION@#An interaction between FLNB and RNF216 was identified through yeast two hybrid experiment. RNF216 may affect the proliferation and migration of GnRH neurons by regulating FLNB or FLNB/FLNA heterodimers.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Proteins / Gene Library / Gonadotropin-Releasing Hormone / Two-Hybrid System Techniques / Ubiquitin-Protein Ligases Type of study: Diagnostic study / Screening study Limits: Humans Language: Chinese Journal: Chinese Journal of Medical Genetics Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Proteins / Gene Library / Gonadotropin-Releasing Hormone / Two-Hybrid System Techniques / Ubiquitin-Protein Ligases Type of study: Diagnostic study / Screening study Limits: Humans Language: Chinese Journal: Chinese Journal of Medical Genetics Year: 2021 Type: Article