Distribution of ETV6-ABL Fusion Gene in Hematopoietic Cell Populations of Myeloproliferative Neoplasm / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1242-1246, 2021.
Article
in Zh
| WPRIM
| ID: wpr-888545
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the expression level of ETV6-ABL fusion gene in different cell populations in patients with myeloproliferative neoplasm (MPN) and therapeutic effect of tyrosine kinase inhibitor (TKI).@*METHODS@#A 42-year-old man who presented with fever, marked leukocytosis and chronic myelogenous leukemia (CML) like MPN was reported. ETV6-ABL fusion gene was detected by real-time PCR and confirmed by direct sequencing. ETV6-ABL mRNA expression in each cell population sorted from peripheral blood by flow cytometry was detected by real-time PCR.@*RESULTS@#ETV6-ABL fusion gene was found out in bone marrow cells and confirmed as type A by direct sequencing. ETV6-ABL fusion gene transcript level in polymorphonuclear cells was nearly 3.6-fold relative to that in total cells, which was significantly higher than that in T cell, B cell and monocyte subsets. The complete blood count (CBC) returned to normal level after treatment with imatinib (400 mg) daily for three months. After TKI treatment for 6 months, the ratio of ETV6-ABL/ABL decreased from 174.1% to 1.9%.@*CONCLUSION@#ETV6-ABL fusion gene positive MPN may have a CML clinical presentation and is sensitive to TKI. ETV6-ABL fusion gene is specifically expressed in polymorphonuclear cells.
Full text:
1
Index:
WPRIM
Main subject:
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Genes, abl
/
Fusion Proteins, bcr-abl
/
Hematopoietic Stem Cell Transplantation
/
Imatinib Mesylate
/
Myeloproliferative Disorders
Limits:
Adult
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2021
Type:
Article