Copy number alteration profiling facilitates differential diagnosis between ossifying fibroma and fibrous dysplasia of the jaws / 国际口腔科学杂志·英文版
International Journal of Oral Science
;
(4): 21-21, 2021.
Article
in English
| WPRIM
| ID: wpr-888698
ABSTRACT
Ossifying fibroma (OF) and fibrous dysplasia (FD) are two fibro-osseous lesions with overlapping clinicopathological features, making diagnosis challenging. In this study, we applied a whole-genome shallow sequencing approach to facilitate differential diagnosis via precise profiling of copy number alterations (CNAs) using minute amounts of DNA extracted from morphologically correlated microdissected tissue samples. Freshly frozen tissue specimens from OF (n = 29) and FD (n = 28) patients were obtained for analysis. Lesion fibrous tissues and surrounding normal tissues were obtained by laser capture microdissection (LCM), with ~30-50 cells (5 000-10 000 µm
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Nuclear Proteins
/
Fibroma, Ossifying
/
Diagnosis, Differential
/
DNA Copy Number Variations
/
Fibrous Dysplasia of Bone
/
Galactosyltransferases
/
Jaw
/
Neoplasm Recurrence, Local
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
International Journal of Oral Science
Year:
2021
Type:
Article
Similar
MEDLINE
...
LILACS
LIS