Three new polyketides from vasR2 gene over-expressed mutant strain of Verrucosispora sp. NS0172 / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 536-539, 2021.
Article
in En
| WPRIM
| ID: wpr-888782
Responsible library:
WPRO
ABSTRACT
Over-expression of the pathway specific positive regulator gene is an effective way to activate silent gene cluster. In the curret study, the SARP family regulatory gene, vasR2, was over-expressed in strain Verrucosispora sp. NS0172 and the cryptic gene cluster responsible for the biosynthesis of pentaketide ansamycin was partially activated. Two tetraketides (1 and 2) and a triketide (3) ansamycins, together with five known compounds (4-8), were isolated and elucidated from strain NS0172OEvasR2. Their NMR data were completely assigned by analysis of their HR-ESI-MS and
Key words
Full text:
1
Index:
WPRIM
Main subject:
Multigene Family
/
Rifabutin
/
Polyketides
/
Micromonosporaceae
Language:
En
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2021
Type:
Article