Evaluation of a Rapid Detection Influenza Virus A Antigens Kit Using Paired Serum Antibody Test
Yonsei Medical Journal
;
: 476-479, 2013.
Article
in English
| WPRIM
| ID: wpr-89559
ABSTRACT
PURPOSE:
To evaluate the feasibility for gold immunochromatographic assay (GICA) in rapid detection of influenza virus A infection. MATERIALS ANDMETHODS:
Seventy-three patients were enrolled. All patients contributed nasopharyngeal secretions and paired serum samples. Nasopharyngeal secretions was used for colloidal gold immunochromatographic rapid assay for influenza A virus immediately after the collection of specimen. Paired serum samples were used for the hemagglutination inhibition assay at the Centers for Disease Control and Prevention influenza network laboratory in Beijing.RESULTS:
Compare GICA test to hemagglutination inhibition (HI) assay, the Kappa value was 0.402 and the p value in the paired chi2 test was higher than 0.05. Therefore, the difference was not statistically significant. The sensitivity of GICA was 50.0% and the specificity was 90.2%, and the negative predictive value was 90.2%.CONCLUSION:
The sensitivity for Influenza A antigen detection by using GICA is relatively low, the specificity is relatively satisfactory.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Influenza A virus
/
Reagent Kits, Diagnostic
/
Chromatography, Affinity
/
Sensitivity and Specificity
/
Gold Colloid
/
Influenza, Human
/
Antigens, Viral
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
English
Journal:
Yonsei Medical Journal
Year:
2013
Type:
Article
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