The Combination of Concentrated Growth Factor and AdiposeDerived Stem Cell Sheet Repairs Skull Defects in Rats
Tissue Engineering and Regenerative Medicine
;
(6): 905-913, 2021.
Article
in English
| WPRIM
| ID: wpr-896402
ABSTRACT
BACKGROUND@#The goal of this study was to create a biomaterial which combines concentrated growth factor (CGF) with an adipose-derived stem cell (ADSC) sheet to promote the repair of skull defects in rats. @*METHODS@#We determined the optimal concentration of CGF extract by investigating the effects of different concentrations (0, 5%, 10%, and 20%) on the proliferation and differentiation of ADSCs. Then we created a complex combining CGF with an ADSC sheet, and tested the effects on bone repair in four experimental rat groups (A) control; (B) ADSC sheet; (C) CGF particles; (D) combination of CGF ? ADSCs. Eight weeks after the procedure, osteogenesis was assessed by micro-CT and hematoxylin and eosin staining. @*RESULTS@#We found that the concentration of CGF extract that promoted optimal ADSC proliferation and differentiation in vitro was 20%. In turn, bone regeneration was promoted the most by the combination of CGF and ADSCs. @*CONCLUSION@#In this study, we determined the optimal ratio of CGF and ADSCs to be used in a biomaterial for bone regeneration. The resulting CGF/ADSCs complex promotes maxillofacial bone defect repair in rats.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Tissue Engineering and Regenerative Medicine
Year:
2021
Type:
Article
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