Identification and characterization of peroxisome proliferator response element in the mouse GLUT2 promoter
Experimental & Molecular Medicine
;
: 101-110, 2005.
Article
in English
| WPRIM
| ID: wpr-90142
ABSTRACT
In the present study, we show that the expression of type 2 glucose transporter isoform (GLUT2) could be regulated by PPAR-gamma in the liver. Rosiglitazone, PPAR-gamma agonist, activated the GLUT2 mRNA level in the primary cultured hepatocytes and Alexander cells, when these cells were transfected with PPAR-gamma/RXR-alpha. We have localized the peroxisome proliferator response element in the mouse GLUT2 promoter by serial deletion studies and site-directed mutagenesis. Chromatin immunoprecipitation assay using ob/ob mice also showed that PPAR-gamma rather than PPAR-alpha binds to the -197/-184 region of GLUT2 promoter. Taken together, liver GLUT2 may be a direct target of PPAR-gamma ligand contributing to glucose transport into liver in a condition when PAPR-gamma expression is increased as in type 2 diabetes or in severe obesity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Monosaccharide Transport Proteins
/
Mice, Transgenic
/
Cells, Cultured
/
Gene Expression Regulation
/
Mutagenesis, Site-Directed
/
Promoter Regions, Genetic
/
Genes, Reporter
/
Protein Isoforms
/
Response Elements
/
Hepatocytes
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2005
Type:
Article
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