B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells
Journal of Korean Medical Science
;
: 391-396, 2006.
Article
in English
| WPRIM
| ID: wpr-9058
ABSTRACT
We investigated the characteristic features of cervical lymph node B cells to determine whether their behavior differs from that of B cells located elsewhere, because cervical lymph nodes may be exposed to continual antigenic stimulation from the naso- and/or oropharynx. B cells were isolated from cervical lymph nodes, spleen and peritoneal fluid of mice, cultured in medium, and exposed to various stimuli. The expression of various surface molecules characteristic of lymphoid B cells was assayed by flow cytometry, and immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. B220+ cells were cultured in medium alone or with lipopolysaccharide, and their entrance into S phase in response to stimuli was measured by proliferative assays. Phenotypic characteristics of cervical lymph node B cells included CD5low, CD23high, CD43low, B7.1low, B7.2low, and Syndecan-1low. Unstimulated lymphoid B cells did not secrete immunoglobulin, but, upon stimulation, secretion of IgM was increased more than secretion of IgA and IgG. B cells actively entered S phase after 48 hr stimulation. These results show that B cells in cervical lymph nodes are conventional B2 cells, like splenic B cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Spleen
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
B-Lymphocytes
/
Cell Culture Techniques
/
Culture Media
/
Cell Proliferation
/
Flow Cytometry
/
Lymph Nodes
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2006
Type:
Article
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