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Identification of Chemical Constituents in Chaishi Tuire Granules by UPLC-ESI-Q-TOF-MS/MS / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 152-159, 2021.
Article in Chinese | WPRIM | ID: wpr-906001
ABSTRACT

Objective:

To rapidly identify the chemical constituents of Chaishi Tuire granules by ultra-performance liquid chromatography-electrospray/quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS).

Method:

Chromatographic separation was conducted on a Phenomenex<sup>®</sup> Luna omega C<sub>18</sub> column (2.1 mm×100 mm, 1.6 μm) with 0.1% formic acid aqueous solution (A)-acetonitrile (B) as the mobile phases for gradient elution (0-20 min, 5%-40%B; 20-40 min, 40%-95%B; 40-43 min, 95%B), the flow rate was set at 0.3 mL·min<sup>-1</sup>. MS data were collected in positive and negative ion modes, the scanning range was <italic>m</italic>/<italic>z</italic> 150-1 500 and electrospray ionization (ESI) was employed. The chemical constituents of Chaishi Tuire granules were identified by comparing with the retention time and the mass data of the reference substances, as well as the accurate mass, MS/MS fragment ionsmass spectrometry databases (PubChem, MassBank, ChemicalBook and others) and related literature.

Result:

A total of 85 chemical constituents were identified, including 28 flavonoids, 24 phenylpropanoids, 11 terpenoids, 10 alkaloids, 4 quinones, and 8 others. Among them, 19 constituents derived from Lonicerae Japonicae Flos, 14 constituents derived from Scutellariae Radix, 10 constituents derived from Isatidis Radix, 9 constituents derived from Taraxaci Herba, 9 constituents derived from Forsythiae Fructus, 4 constituents derived from Bupleuri Radix, 4 constituents derived from Anemarrhenae Rhizoma, and 4 constituents derived from Rhei Radix et Rhizoma.

Conclusion:

Chaishi Tuire granules is rich in phytochemicals, which are derived from many of traditional Chinese medicines. This study can lay a foundation for the quality control, material basis and <italic>in vivo</italic> metabolic analysis of this preparation.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2021 Type: Article