Curcumin reduces cold storage-induced damage in human cardiac myoblasts
Experimental & Molecular Medicine
;
: 139-148, 2007.
Article
in English
| WPRIM
| ID: wpr-90619
ABSTRACT
Curcumin is a polyphenolic compound possessing interesting anti-inflammatory and antioxidant properties and has the ability to induce the defensive protein heme oxygenase-1 (HO-1). The objective of this study was to investigate whether curcumin protects against cold storage-mediated damage of human adult atrial myoblast cells (Girardi cells) and to assess the potential involvement of HO-1 in this process. Girardi cells were exposed to either normothermic or hypothermic conditions in Celsior preservation solution in the presence or absence of curcumin. HO-1 protein expression and heme oxygenase activity as well as cellular damage were assessed after cold storage or cold storage followed by re-warming. In additional experiments, an inhibitor of heme oxygenase activity (tin protoporphyrin IX, micrometer) or siRNA for HO-1 were used to investigate the participation of HO-1 as a mediator of curcumin- induced effects. Treatment with curcumin produced a marked induction of cardiac HO-1 in normothermic condition but cells were less responsive to the polyphenolic compound at low temperature. Cold storage-induced damage was markedly reduced in the presence of curcumin and HO-1 contributed to some extent to this effect. Thus, curcumin added to Celsior preservation solution effectively prevents the damage caused by cold- storage; this effect involves the protective enzyme HO-1 but also other not yet identified mechanisms.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Cryopreservation
/
Gene Expression Regulation, Enzymologic
/
Cell Survival
/
Cells, Cultured
/
Cell Death
/
Cold Temperature
/
Cryoprotective Agents
/
Curcumin
/
Myoblasts, Cardiac
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2007
Type:
Article
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