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Analysis on Chemical Constituents in Rhizomes of Bergenia scopulosa by UHPLC-Q Exactive Focus MS/MS / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 118-128, 2021.
Article in Chinese | WPRIM | ID: wpr-906464
ABSTRACT

Objective:

Ultra-high performance liquid chromatography coupled with quadrupole/electrostatic field orbital trap high resolution mass spectrometry (UHPLC-Q Exactive Focus MS/MS) was developed to rapidly analyze and identify the chemical components in the rhizomes of <italic>Bergenia scopulosa</italic>.

Method:

The 75% methanol extract of <italic>B</italic>.<italic> scopulosa</italic> rhizomes was analyzed on a Thermo Accucore aQ RP18 column (2.1 mm×150 mm, 2.6 μm) with the mobile phase of methanol (A) and 0.1% formic acid aqueous solution (B) for gradient elution (0-40 min 5%-95%A, 40-45 min 95%A), the flow rate was 0.3 mL·min<sup>-1</sup> and the column temperature was at 30 ℃. The information of the chemical constituents was acquired in positive and negative ion modes by heated electrospray ion source (HESI), and the scanning range was <italic>m</italic>/<italic>z</italic> 80-1 200.

Result:

A total of 66 chemical constituents were identified, including 2 free amino acids, 7 bergenin derivatives, 15 flavonoids, 15 organic acids, 25 glycosides, and 2 others.

Conclusion:

The chemical constituents in the rhizomes of <italic>B</italic>.<italic> scopulosa</italic> can be identified systematically, accurately and rapidly by this method. Among them, 8 compounds were unambiguously identified by comparing with reference substances (succinic acidarbutingallic acid, protocatechuic acid, bengenin, catechinchlorogenic acid and caffeic acid), 51 compounds were found from <italic>B</italic>.<italic> scopulosa</italic> for the first time and 28 compounds were found from the genus <italic>Bergenia</italic> for the first time. This paper can provide an important basis for the further material basis clarification and quality assessment of <italic>B</italic>.<italic> scopulosa</italic>.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2021 Type: Article