Effect of Fuzheng Kangai Decoction on Immune Regulation and Inhibition of Tumor Growth in Rats with Ovarian Carcinoma / 中国实验方剂学杂志

ABSTRACT

Objective:To explore the effect of Fuzheng Kangai decotion （FZKAD） on the immune regulation and the inhibition of tumor growth in rats with ovarian carcinoma. Method:Kunming mice were randomly divided into normal group， lentinan （0.05 g·kg^-1） group， and high （27.3 g·kg^-1）， medium （13.65 g·kg^-1） and low （6.825 g·kg^-1） dose groups of FZKAD， with 10 mice in each group， serum hemolytic value （HC₅₀）， antibody-forming cells and the phagocytosis of mononuclear macrophages were measured. Fischer 344 rat xenograft model was established through inoculation of NUTU-19 cell in the right axilla， and the model rats were randomly divided into model group， cisplatin group （0.002 g·kg^-1）， and high （18.9 g·kg^-1）， medium （9.45 g·kg^-1）， and low （4.725 g·kg^-1） dose groups of FZKAD， with 10 rats in each group， in addition， 10 healthy rats were randomly selected as the normal group. Tumor quality， tumor inhibition rate， T lymphocyte subsets， and expressions of serum cytokines， enhancer binding protein homologous protein 1（XBP1） and enhancer binding protein homologous protein （CHOP） protein in tumor tissues were detected after 14 days of administration. Result:Compared with normal group， HC₅₀， level of antibody-forming cells， phagocytic index and phagocytic activity of mice in high， medium and low-dose groups of FZKAD were significantly increased （<italic>P</italic><0.05， <italic>P</italic><0.01）. Tumor quality and XBP1 protein expression in high， medium and low-dose groups of FZKAD were significantly decreased （<italic>P</italic><0.01） compared with the model group， while the tumor inhibition rate， CD4⁺， CD8⁺ T cell ratio， CD4⁺/CD8⁺ ratio， tumor necrosis factor-<italic>α</italic> （TNF-<italic>α</italic>）， interleukin-2 （IL-2）， <italic>γ-</italic>interferon （IFN-<italic>γ</italic>） expression and CHOP protein expression were significantly increased （<italic>P</italic><0.05， <italic>P</italic><0.01）. Conclusion:FZKAD can improve the immune function of normal mice and inhibit the tumor growth in rats with ovarian carcinoma， and the immunity regulation effect is the main mechanism.