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Effects of Quercetin on the Expression of Ang Ⅱ-induced Myocardial Contractile Protein of Rats through ACE 2- Ang-(1-7)-Mas Axis / 中国药房
China Pharmacy ; (12): 2839-2845, 2021.
Article in Chinese | WPRIM | ID: wpr-906649
ABSTRACT
OBJECTIVE:To in vestigate the effects of quercetin (Que)on the expressio n of angiotensin Ⅱ(AngⅡ)-induced myocardial contractile proteins of primary rats through angiotensin-converting enzyme 2-angiotensin-(1-7)-Mas (ACE2-Ang- (1-7)-Mas)axis. METHODS :Cardiac tissue of rats aged 1-2 d were collected ,and primary cardiomyocytes were isolated and cultured. The gene silencing model of cardiomyocytes ACE2 was constructed. Experiments were divided into 12 groups. Among them,AngⅡ group,AngⅡ+ small interference RNAsiRNA)group,and Ang Ⅱ+ A 779 group were the model groups ;AngⅡ+ losartan group was positive control group ;AngⅡ+Que40 group,AngⅡ+Que80 group,AngⅡ+siRNA+Que40 group,AngⅡ+ siRNA+Que80 group,AngⅡ+A779+Que40 group and Ang Ⅱ+A779+Que80 group were the experimental groups ;blank group and siRNA group were set up. Ang Ⅱ concentration was 1×10-6 mol/L;siRNA final concentration was 50 nmol/L;Que concentration was 40 and 80 μmol/L;A779(Mas receptor inhibitor )concentration was 1 μmol/L;losartan concentration was 1×10-4 mol/L. mRNA and protein expression of ACE 2,Ang-(1-7) and Mas in primary cardiomyocytes were detected ;the expressions of myocardial contractile proteins were also determined ,such as Na +/Ca2+ exchange channel (NCX),calcium pump (SERCA2a), phosphoprotein (PLB). RESULTS :Compared with Ang Ⅱ group,mRNA expression of Mas was increased significantly in Ang Ⅱ + Que 80 group (P<0.05);mRNA expression of ACE2 and Mas were increased significantly in Ang Ⅱ + CZ0210-01) losartan group (P<0.05). Compared with Ang Ⅱ group,the 851136165@qq.com protein expression of ACE 2 and Ang- (1-7) were increased significantly in Ang Ⅱ+ Que 40 group(P<0.05);compared with Ang Ⅱ + siRNA group ,the protein expression of Ang-(1-7)were increased significantly in Ang Ⅱ+ siRNA+Que 40 group(P<0.05);compared with Ang Ⅱ+A779 group,the protein expression of Ang- (1-7)were increased significantly in Ang Ⅱ+A779+ Que 40 group(P<0.05). Compared with Ang Ⅱ group,the protein expression of NCX was decreased in Ang Ⅱ+Que40 group(P<0.05),protein expression of NCX was reduced in Ang Ⅱ+ losartan group (P<0.05);compared with Ang Ⅱ+A779 group,the protein expression of NCX was decreased in Ang Ⅱ+A779+ Que80 group (P<0.05). CONCLUSIONS :Que improves the expression of Ang Ⅱ -induced ACE 2-Ang-(1-7)-Mas axis in cardiomyocyte model to some extent ,so as to regulate myocardial contractile protein.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2021 Type: Article