Correlation between miR-206 expression and Wnt/β-catenin pathway in peripheral blood mononuclear cells of patients with hyperthyroidism / 中华地方病学杂志

ABSTRACT

Objective:To explore the correlation between the expression level of microRNA (miR)-206 and the expression levels of Wnt-1 and β-catenin in Wnt/β-catenin pathway in peripheral blood mononuclear cells of patients with hyperthyroidism.Methods:Using a prospective design, 79 patients with hyperthyroidism admitted to Wuwei City People's Hospital from January 2018 to June 2020 were selected as observation group, and 70 cases of healthy physical examination in Wuwei City People's Hospital from January 2019 to September 2020 were selected as control group. The observation group was treated with anti-thyroid drugs, and tambazole was orally taken 10-20 mg/d during the treatment period; after the serum thyroid hormone was normal, the dosage was reduced and maintained at 5-10 mg/d for 12 months. Quantitative real-time PCR (qRT-PCR) was used to detect the expression levels of miR-206, Wnt-1 and β-catenin mRNA in peripheral blood mononuclear cells in observation group before and after treatment and control group.Results:The expression level of miR-206 in peripheral blood mononuclear cells of observation group before treatment was lower than that of control group (0.28 ± 0.07 vs 0.79 ± 0.15), and the expression levels of Wnt-1 and β-catenin mRNA were higher than those of control group (6.75 ± 1.39 vs 2.23 ± 0.65, 5.83 ± 1.24 vs 3.21 ± 0.86, P < 0.05). The expression level of miR-206 (0.54 ± 0.13) in peripheral blood mononuclear cells of observation group after treatment was higher than that before treatment, and the expression levels of Wnt-1 and β-catenin mRNA (3.62 ± 1.08, 4.34 ± 1.16) were lower than those before treatment ( P < 0.05). The expression level of miR-206 in peripheral blood mononuclear cells of patients with hyperthyroidism was negatively correlated with the expression levels of Wnt-1 and β-catenin mRNA ( r = - 0.763, - 0.798, P < 0.05). Conclusions:The expression level of miR-206 in peripheral blood mononuclear cells of patients with hyperthyroidism is low, while the expression levels of Wnt-1 and β-catenin mRNA are high. The expression level of miR-206 is negatively correlated with the expression levels of Wnt-1 and β-catenin mRNA.