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Effects of dexmedetomidine on JNK in A549 cells with hypoxia/reoxygenation injury / 中国临床药理学与治疗学
Chinese Journal of Clinical Pharmacology and Therapeutics ; (12): 1346-1351, 2017.
Article in Chinese | WPRIM | ID: wpr-909301
ABSTRACT

AIM:

To investigate the effects and c-Jun N-terminal kinase(JNK) expression of dexmedetomidine (DEX) on A549 cells with hypoxia/reoxygenation (H/R) injury.

METHODS:

A549 cells were cultivated and were randomly divided into four groups (n =10)control group (N),DEX group (D),hypoxia/reoxygenation injury group (H),hypoxia/reoxygenation injury + DEX interfere group (HD).After all models were completed,the morphological changes of A549 cells were observed under the inverted microscope.Cell activity was detected by CCK-8 and the apoptosis index (AI) was detected by in situ end labeling (TUNEL) method.The expression of GRP78,p-JNK,caspase-3 at protein levels and GRP78,JNK mRNA were detected by Western blot and RT-PCR.

RESULTS:

Compared with N group,the number of adherent cells in H group decreased significantly,and cell morphology changed.The expression of OD value in H group decreased obviously (P < 0.01),the expression of AI value,GRP78,p-JNK,caspase-3 protein and GRP78,JNK mRNA were significantly increased (P< 0.01).HD group compared with H group,the cell damage alleviated,the expression of OD value was increased (P < 0.01),the number of apoptosis cells and the AI value in HD group were significantly decreased (P < 0.01).Dramatically decreased the expression of p-JNK,caspase-3 protein and JNK mRNA (P < 0.01).

CONCLUSION:

DEX can effectively alleviate A549 cells damage induced by H/R injury,which may be related to inhibition of the JNK pathway.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Clinical Pharmacology and Therapeutics Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Clinical Pharmacology and Therapeutics Year: 2017 Type: Article