Effect and mechanism of circ-EIF3I on the growth and metastasis of hepatocellular carcinoma / 中华肝胆外科杂志

ABSTRACT

Objective:To investigate the function of circ-EIF3I on the growth and metastasis of hepatocellular carcinoma (HCC) and its possible mechanism.Methods:A total of 39 HCC patients admitted to the Fourth Hospital of Hebei Medical University from May 2014 to October 2015 were selected as the study subjects, including 29 males and 10 females, aged (62.2±5.6) years old. Part of HCC tissues and adjacent tissues were obtained during the surgical operation. Fluorescence quantitative PCR was used to detect the expression of circ-EIF3I, and Western blotting was used to detect phosphoglycerate kinase 1 (PGK1) in HCC and adjacent tissues, respectively. Small molecule RNA interference and gene overexpression experiments were used to adjust its expression in Hep3B and Huh-7 HCC cell lines, and then MTT cell viability test was used to detect cell proliferation ability. Transwell assaya was used to detect cell migration and invasion ability. Finally, the dual luciferase report experiment was used to detect the targeting relationship between circ-EIF3I and miR-149-5p/miR-1271-5p, and the targeting relationship between miR-149-5p/miR-1271-5p and PGK1.Results:Compared with adjacent tissues, the relative expression of circ-EIF3I [(4.32±0.62) vs. (1.24±0.59)] and PGK1 [(2.69±0.19) vs. (1.00±0.07)] in HCC tissues from 39 cases were increased, and the differences were statistically significant (all P<0.05). Compared with the negative control group, the cell viability of the circ-EIF3I small molecule interfering RNA group was reduced [Hep3B (55.3±7.5)% vs. (100.0±9.2)%; Huh-7 (42.7±6.0)% vs. (100.0±5.6)%] , the number of migrating cells was decreased [Hep3B (71.0±10.0) vs. (130.0±15.0); Huh-7 (50.0±8.5) vs. (125.0±10.0)], the number of invasive cells also was decreased [Hep3B (52.0±7.0) vs. (105.0±13.0); Huh-7 (60.0±8.0) vs. (144.0±11.0)], the difference was statistically significant (all P<0.05). The dual luciferase report experiment confirmed that circ-EIF3I could target miR-149-5p/miR-1271-5p, and miR-149-5p/miR-1271-5p can target PGK1. Over expression of PGK1 could significantly reverse the effects of knockdown of circ-EIF3I on the proliferation, migration and invasion of HCC cells. Conclusion:Knockdown of circ-EIF3I could inhibit the proliferation, migration, invasion of HCC cells by regulating the miR-149-5p/miR-1271-5p/PGK1 molecular axis.