HLA-restricted and Antigen-specific CD8+ T Cell Responses by K562 Cells Expressing HLA-A*0201
Immune Network
;
: 179-184, 2006.
Article
in English
| WPRIM
| ID: wpr-91160
ABSTRACT
BACKGROUND:
Identification of antigen-specific T cells has yielded valuable information on pathologic process and the disease state. Assays for quantification of inflammatory cytokines or lytic-granule molecules have been generally used to evaluate antigen specific T cell response, however their applicability have been hampered due to the limited source of autologous antigen-presenting target cells (APC).METHODS:
K562, a leukemic cell line deficient of human leukocyte antigen (HLA), was transfected with a gene encoding HLA-A*02 (K562/A*02) and its function as stimulator cells in inducing activation of HLA-matched T cells was evaluated by IFN-gamma enzyme linked immunospot (ELISPOT) assay.RESULTS:
The stable transfectant K562/A*02 pulsed with HLA- A*02 restricted peptide could specifically induce IFN-gamma secretion by CD8+ T cells compared to no detectable secretion by CD4+ T cells. However, CD56+ NK cells secreted IFN-gamma in both K562/A*02 with peptide and without peptide. The number of IFN-gamma secreted CD8+ T cells was increased according to the ratio of T cells to K562 and peptide concentration. Formalin-fixed K562/A*02 showed similar antigen presenting function to live K562/A*02. Moreover, K562/A*02 could present antigenic- peptide to not only A*0201 restricted CD8+ T cells but also CD8+ T cells from A*0206 donor.CONCLUSION:
These results suggest that K562/A*02 could be generally used as target having specificity and negligible background for measuring CD8+ T cell responses and selective use of K562 with responsder matched HLA molecules on its surface as APC may circumvent the limitation of providing HLA-matched autologous target cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Tissue Donors
/
Killer Cells, Natural
/
T-Lymphocytes
/
Cell Line
/
Cytokines
/
Genes, vif
/
Sensitivity and Specificity
/
K562 Cells
/
Leukocytes
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Immune Network
Year:
2006
Type:
Article
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