Evaluation of the quality of the IgM antibody capture ELISA diagnostic kit for the Japanese encephalitis virus produced by National Institute of Hygiene and Epidemiology
Journal of Preventive Medicine
;
: 55-59, 2008.
Article
in Vietnamese
| WPRIM
| ID: wpr-912
ABSTRACT
Background:
IgM antibody capture ELISA (MAC-ELISA) technique has been widely applied for Japanese Encephalitis Virus (JEV) diagnosis. So far rare internationally commercial kits are available. Thus, the international evaluation of the kit is required as per the recommendation of the WHO.Objectives:
To evaluate the quality of the IgM antibody capture ELISA diagnostic kit for JEV produced by the Vietnam National Institute of Hygiene and Epidemiology (NIHE). Subjects andmethod:
In this study, NIID kit was used as control to check the kit from NIHE. Both NIHE and NIID kits were used to detect JEV IgM among 38 serum and 6 CFS samples, which belongs to 5 sample groups (JE patients group, dengue patients group, other viral encephalitis patients group, Tick Born Encephalitis (TBE) patient group and healthy JE vaccinated donors group).Results:
The detection of JEV IgM by NIHE kit was concurrent with the NIID kit. There is no positive with the JE in the groups of Dengue patients, TBE, other virus encephalitis patients and JE vaccinated donors.Conclusion:
MAC-ELISA kit of NIHE can be used for different diagnosis of JEV and Dengue virus (both viruses are in Flavivirus genus), as well as other viruses caused by encephalitis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoglobulin M
/
Encephalitis Virus, Japanese
Type of study:
Diagnostic study
/
Practice guideline
/
Screening study
Language:
Vietnamese
Journal:
Journal of Preventive Medicine
Year:
2008
Type:
Article
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