Establishment of reverse transcription polymerase chain reaction for detection of Getah virus infection in livestock
Korean Journal of Veterinary Research
;
: 37-42, 2017.
Article
in English
| WPRIM
| ID: wpr-91209
ABSTRACT
Getah virus (GETV) infection causes sporadic outbreaks of mild febrile illness in horses and reproductive failure in pigs. In this study, we established a reverse transcription polymerase chain reaction (RT-PCR) method to detect GETV from suspected virus-infected samples. The reaction conditions were optimized and validated by using RNA extracted from GETV propagated in cell culture. A GETV-specific GED4 primer set was designed and used to amplify a 177 bp DNA fragment from a highly conserved region of the E1 glycoprotein gene in the GETV genome. RT-PCR performed with this primer set revealed high sensitivity and specificity. In the sensitivity test, the GED4 primer set detected GETV RNA at the level of 10(2.0) TCID₅₀/mL. In the specificity test, the GED4 primer set amplified only a single band of PCR product on the GETV RNA template, without non-specific amplification, and exhibited no cross-reactivity with other viral RNAs. These results suggest that this newly established RT-PCR method is useful for accurate identification of GETV infection in animals.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
DNA
/
RNA
/
RNA, Viral
/
Glycoproteins
/
Polymerase Chain Reaction
/
Disease Outbreaks
/
Sensitivity and Specificity
/
Genome
/
Alphavirus
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Korean Journal of Veterinary Research
Year:
2017
Type:
Article
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