Your browser doesn't support javascript.
loading
Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
Article in English | WPRIM | ID: wpr-91220
Responsible library: WPRO
ABSTRACT
In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Rabbits / Female / Ixodidae / Electrophoresis, Polyacrylamide Gel / Tandem Mass Spectrometry / Arthropod Proteins / Animals / Molecular Weight / Antigens Type of study: Screening study Language: English Journal: The Korean Journal of Parasitology Year: 2015 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Rabbits / Female / Ixodidae / Electrophoresis, Polyacrylamide Gel / Tandem Mass Spectrometry / Arthropod Proteins / Animals / Molecular Weight / Antigens Type of study: Screening study Language: English Journal: The Korean Journal of Parasitology Year: 2015 Type: Article