Diagnostic value of serum miRNA-145, miRNA-21 and miRNA-197 for non-small cell lung cancer / 肿瘤研究与临床

ABSTRACT

Objective:To explore the diagnostic value of serum miRNA-145 (miR-145), miRNA-21 (miR-21) and miRNA-197 (miR-197) for non-small cell lung cancer (NSCLC).Methods:The clinical data of 65 NSCLC patients in Luzhou Hospital of Traditional Chinese Medicine in Sichuan Province from January 2019 to June 2020 were retrospectively analyzed, and 60 healthy physical examiners were selected as the healthy control group. The relative expressions of serum miR-145, miR-21 and miR-197 in all subjects were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and their relationship with clinicopathological features of NSCLC patients was analyzed. The receiver operating characteristic (ROC) curve was used to analyze the efficacy of miR-145, miR-21 and miR-197 alone and in combination in the diagnosis of NSCLC.Results:The relative expression of miR-145 in the NSCLC group was lower than that in the healthy control group (1.05±0.40 vs. 1.38±0.44, t = -4.326, P < 0.01), and the relative expressions of miR-21 and miR-197 were higher than those in the control group (2.37±0.89 vs. 0.83±0.25, 4.42±0.75 vs. 1.10±0.33, both P < 0.01). In the NSCLC group, the relative expressions of miR-21 and miR-197 in patients with TNM stage Ⅲ-Ⅳ were higher than those in patients with TNM stage Ⅰ-Ⅱ (both P < 0.01), and the relative expression of miR-145 in patients with adenocarcinoma was higher than that in patients with squamous cell carcinoma ( P < 0.01), and the relative expression of miR-21 in patients with lymph node metastasis was higher than that in patients without lymph node metastasis ( P < 0.01). When miR-145, miR-21 and miR-197 were used alone to diagnose NSCLC, the area under the curve (AUC) was 0.842 (95% CI 0.795-0.907), 0.868 (95% CI 0.812-0.938) and 0.857 (95% CI 0.801-0.913), and the best diagnostic cut-off values ??were 1.14, 2.03 and 2.98. When the best cut-off value was used for diagnosis, miR-145 alone was the most sensitive (83.76%), and miR-21 alone was the most specific (87.63%). The AUC for the combined detection of the three was 0.939 (95% CI 0.904-0.965), the diagnostic sensitivity was 91.58%, and the specificity was 79.86%. Conclusions:The relative expressions of miR-145, miR-21 and miR-197 are related to the pathological type, TNM staging and lymph node metastasis of NSCLC patients, and they may become markers for the auxiliary diagnosis of NSCLC. The combined detection of the three can improve the diagnostic efficiency and sensitivity.