Identification of mono- or poly-specific monoclonal antibody to Porphyromonas gingivalis heat-shock protein 60
Journal of Periodontal & Implant Science
;
: 54-59, 2011.
Article
in English
| WPRIM
| ID: wpr-91748
ABSTRACT
PURPOSE:
The aim of this study was to define the immunoreactive specificity of Porphyromonas gingivalis (P. gingivalis) heat shock protein (HSP) 60 in periodontitis and atherosclerosis.METHODS:
In an attempt to define the cross-reactive bacterial heat-shock protein with human self-antigen at molecular level, we have introduced a novel strategy for cloning hybridoma producing anti-P. gingivalis HSP 60 which is polyreactive to bacterial HSPs or to the human homolog.RESULTS:
Five cross-reactive clones were obtained which recognized the #19 peptide (TLVVNRLRGSLKICAVKAPG) among 37 synthetic peptides (20-mer, 5 amino acids overlapping) spanning the whole molecule of P. gingivalis HSP 60. We have also established three anti-P. gingivalis HSP 60 monoclonal antibodies demonstrating mono-specificity. These clones recognized the #29 peptide (TVPGGGTTYIRAIAALEGLK).CONCLUSIONS:
Peptide #19 and #29 of P. gingivalis HSP 60 might be important immunoreactive epitopes in the immunopathogenic mechanism of bacterial antigen-triggered autoimmune diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Periodontitis
/
Autoimmune Diseases
/
Sensitivity and Specificity
/
Clone Cells
/
Porphyromonas
/
Porphyromonas gingivalis
/
Chaperonin 60
/
Cloning, Organism
/
Amino Acids
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Journal of Periodontal & Implant Science
Year:
2011
Type:
Article
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