Comparison study of the response with botulinum toxin muscle injection in the ICR mice from three different sources / 한국실험동물학회지

ABSTRACT

Botulinum-toxin A (BoNT/A) is a widely used not only for cosmetics but also for various experimental purposes including muscle-related research. In this study, we applied BoNT/A to mouse muscle of three different sources to compare and evaluate the biological and pathological response. The three different mouse sources consist of Korl ICR (Korea FDA source), AICR (USA source) and BICR (Japan source) which were purchased from each different vendors. To compare the responses of ICR mice with BoNT/A muscle injection, we examined the body weight, hematological and serum biochemistry analysis. Also, we evaluated the muscle change by histopathological analysis and gene expression patterns of muscle-related target by qPCR. The body weight gain was decreased in the BoNT/ A-treated group compared with the control group. In clinical pathologic analysis and gene expression patterns, the data showed that the responses in the BoNT/A-treated group were similar compared with the control group. Decreased muscle fiber was observed in BoNT/A-treated group compared with control group, while KorlICR showed a little low response with the other mouse sources. In conclusion, our results suggest that three different sources ICR mice (KorlICR, AICR and BICR) have a similar biological and pathological responses in BoNT/A muscle injection.