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Experimental Study on Separation of Fetal Nucleated Red Blood Cell from the Whole Blood Using Microfluidic Chip / 医用生物力学
Journal of Medical Biomechanics ; (6): E903-E909, 2021.
Article in Chinese | WPRIM | ID: wpr-920701
ABSTRACT
Objective To seperate fetal nucleated red blood cells (fNRBCs) from the whole maternal peripheral blood effectively by designing a circular channel microfluidic chip. Methods A microfluidic chip is designed by utilizing the margination in blood flow and the specific adhesion characteristics of immuno-agent anti-CD147. With the whole umbilical cord blood, the effects of different shear forces on the enrichment of fNRBCs was studied by immunofluorescence counting. Results Increasing shear rate in microfluidic chip could improve the number of captured fNRBCs compared with the static adhesion. With the increase of shear rate of blood flow, the number of the captured cells increased at first, and then decreased. Conclusions The use of microfluid chip can effectively seperate fNRBCs from the whole blood. The results provide an experimental reference for the non-invasive prenatal diagnosis research and the exploration on the mechanism of fetal cell migration.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Medical Biomechanics Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Medical Biomechanics Year: 2021 Type: Article