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Identification of phosphatidic acid interacting proteins in Ganoderma lingzhi / 生物工程学报
Chinese Journal of Biotechnology ; (12): 3293-3299, 2021.
Article in Chinese | WPRIM | ID: wpr-921425
ABSTRACT
Ganoderma lingzhi is widely recognized as a medicinal basidiomycetes. Triterpene acids (TAs) are the key bioactive medicinal components of G. lingzhi. Our previous studies have shown that phospholipid acid (PA) produced by phospholipase D (PLD) plays a regulatory role in TA synthesis. In order to further elucidate the molecular mechanism how PA regulates TA synthesis in G. lingzhi, PA beads enrichment combined with LC-MS/MS technology was used to identify PA interacting proteins in G. lingzhi. A total of 19 PA interacting proteins were identified, including cytochrome P450 monooxygenase (GL22084), specific protein kinase MAPK (GL23765), catalase and cell surface hydrophobicity-associated protein. GST tagged GL22084 and GL23765 proteins were obtained through gene cloning, heterologous expression, and purification. The interactions between GL22084/GL23765 and PA were verified by GST pull down assay. The identification of PA interacting proteins provides a basis for further understanding the molecular mechanism how PLD-mediated PA signaling molecules regulates the TA synthesis in G. lingzhi. Moreover, the PA interacting proteins identified in this study can also provide clues for the research of PLD/PA signaling pathway in other species.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphatidic Acids / Chromatography, Liquid / Ganoderma / Tandem Mass Spectrometry Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphatidic Acids / Chromatography, Liquid / Ganoderma / Tandem Mass Spectrometry Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article