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Effect of interactions of chromatin regulatory elements with different promoters on the regulation of gene expression / 生物工程学报
Chinese Journal of Biotechnology ; (12): 3310-3322, 2021.
Article in Chinese | WPRIM | ID: wpr-921427
ABSTRACT
The effect of altering the promoter region of ubiquitous chromatin-opening element (UCOE) and matrix attachment region (MAR) on stable and efficient expression of genes was investigated. Four different promoters were tested, namely, oct4 containing an enhancer region, sox2 having a CpG island, nanog having no regulatory elements, and CMV containing a CpG island and an enhancer region. Eight reporter plasmids were constructed pOCT4-UCOE, pOCT4-MAR, pSOX2-UCOE, pSOX2-MAR, pNANOG-UCOE, pNANOG-MAR, pCMV-UCOE, and pCMV-MAR. Stable and efficient expression was observed when UCOE combined with the oct4 promoter, whereas the sox2 was the best promoter suited for MAR. Comparison of the stable clones of oct4-UCOE and sox2-MAR showed that UCOE-regulated expression is more stable and efficient than MAR-regulated expression. When CpG island-containing promoter is linked with UCOE, stable and efficient expression could be observed. These data suggest that an enhancer region in the promoter leads to high, yet unstable expression when combined with UCOE, whereas CpG islands stabilize expression. In conclusion, UCOE and MAR interact with regulatory elements on the promoter by altering the chromatin open state and chromatin loop to regulate gene expression.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Chromatin / Gene Expression / Gene Expression Regulation / Promoter Regions, Genetic / CpG Islands Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Chromatin / Gene Expression / Gene Expression Regulation / Promoter Regions, Genetic / CpG Islands Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article