Your browser doesn't support javascript.
loading
Shotgun metagenome sequencing of Chinese gut microbiota: a review / 生物工程学报
Chinese Journal of Biotechnology ; (12): 3717-3733, 2021.
Article in Chinese | WPRIM | ID: wpr-921460
ABSTRACT
The research on the relationship between gut microbiota and human health continues to be a hot topic in the field of life science. Culture independent 16S rRNA gene high-throughput sequencing is the current main research method. However, with the reduction of sequencing cost and the maturity of data analysis methods, shotgun metagenome sequencing is gradually becoming an important method for the study of gut microbiome due to its advantages of obtaining more information. With the support from the human microbiome project, 30 805 metagenome samples were sequenced in the United States. By searching NCBI PubMed and SRA databases, it was found that 72 studies collecting about 10 000 Chinese intestinal samples were used for metagenome sequencing. To date, only 56 studies were published, including 16 related to metabolic diseases, 16 related to infectious and immune diseases, and 12 related to cardiovascular and cerebrovascular diseases. The samples were mainly collected in Beijing, Guangzhou, Shanghai and other cosmopolitan cities, where great differences exist in sequencing platforms and methods. The outcome of most studies are based on correlation analysis, which has little practical value in guiding the diagnosis and treatment of clinical diseases. Standardizing sampling methods, sequencing platform and data analysis process, and carrying out multi center parallel research will contribute to data integration and comparative analysis. Moreover, insights into the functional verification and molecular mechanism by using the combination of transcriptomics, proteomics and culturomics will enable the gut microbiota research to better serve the clinical diagnosis and treatment.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: United States / RNA, Ribosomal, 16S / China / Metagenome / Microbiota / Gastrointestinal Microbiome Limits: Humans Country/Region as subject: North America / Asia Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: United States / RNA, Ribosomal, 16S / China / Metagenome / Microbiota / Gastrointestinal Microbiome Limits: Humans Country/Region as subject: North America / Asia Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article