Catalytic mechanism, molecular engineering and applications of threonine aldolases / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 4215-4230, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-921500
ABSTRACT
Threonine aldolases catalyze the aldol condensation of aldehydes with glycine to furnish β-hydroxy-α-amino acid with two stereogenic centers in a single reaction. This is one of the most promising green methods for the synthesis of optically pure β-hydroxy-α-amino acid with high atomic economy and less negative environmental impact. Several threonine aldolases from different origins have been identified and characterized. The insufficient -carbon stereoselectivity and the challenges of balancing kinetic versus thermodynamic control to achieve the optimal optical purity and yield hampered the application of threonine aldolases. This review summarizes the recent advances in discovery, catalytic mechanism, high-throughput screening, molecular engineering and applications of threonine aldolases, with the aim to provide some insights for further research in this field.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Glycine Hydroxymethyltransferase
/
Substrate Specificity
/
Threonine
/
Kinetics
/
Catalysis
/
Amino Acids
/
Glycine
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article
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