Effects of Signaling Activation of Bone Morphogenetic Protein 2 on the Differentiation and Infiltration of Regulatory T Lymphocytes in Tumors / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 897-904, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-921557
ABSTRACT
Objective To determine whether the signaling activation of bone morphogenetic protein 2(BMP2)can induce myeloid-derived suppressor cells(MDSC)to secret transforming growth factor β(TGF-β),further enhancing the differentiation and infiltration of regulatory T lymphocytes(Treg)into tumor tissue. Methods The BMP2-induced mRNA and protein expression of TGF-β in MDSC was detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA),respectively.The effect of BMP2-induced TGF-β secretion by MDSC on Treg differentiation was then determined by flow cytometry.Finally,we implanted the recombined human bone morphogenetic protein 2(rhBMP2)collagen gels into tumor-burdened mice to examine the role of BMP2 in Treg differentiation via MDSC-secreted TGF-β
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Differentiation
/
Transforming Growth Factor beta
/
T-Lymphocytes, Regulatory
/
Bone Morphogenetic Protein 2
/
Myeloid-Derived Suppressor Cells
/
Neoplasms
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2021
Type:
Article
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