Determination of β-lactoglobulin in Anti-HPV Biological Protein Dressing by Amino Acid Assay / 中国医疗器械杂志
Chinese Journal of Medical Instrumentation
;
(6): 551-554, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-922057
ABSTRACT
OBJECTIVE@#To establish an amino acid assay for the determination of β-lactoglobulin in Anti-HPV biological protein dressing.@*METHODS@#Under acidic conditions, β-lactoglobulin is hydrolyzed into free amino acids, separated by cation exchange chromatography, and derivatived after ninhydrin column. The chromatogram at 570 nm is collected. The content of β-lactoglobulin in the sample is indirectly determined by measuring the lysine content obtained by hydrolysis.@*RESULTS@#β-lactoglobulin has a good linear relationship in the concentration range of 77.28~309.12 μg/mL (@*CONCLUSIONS@#The method is simple, specific, accurate and reproducible, which is suitable for the quantitative analysis of β-lactoglobulin in anti-HPV biological protein dressing.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bandages
/
Amino Acids
/
Lactoglobulins
Language:
Chinese
Journal:
Chinese Journal of Medical Instrumentation
Year:
2021
Type:
Article
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