Extract from Zanthoxylum piperitum Induces Apoptosis of AGS Gastric Cancer Cells Through Akt/MDM2/p53 Signaling Pathway / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 752-759, 2021.
Article
in En
| WPRIM
| ID: wpr-922116
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To determine the effect of Zanthoxylum piperitum extracet (ZPE) on apoptosis and analyze anticancer substances in ZPE, changes in proteins related to apoptosis, and pathological changes in tumors in mouse.@*METHODS@#Fifteen 4-week-old female BALB/c nu/nu mice were divided into 3 groups depending on ZPE dose, with 5 in each group. AGS gastric carcinoma cells (1 × 10@*RESULTS@#High performance liquid chromatography (HPLC) analysis showed that ZPE contained organic sulfur compounds such as alliin and S-allylcysteine. MTT assay results revealed that ZPE (10-85 µ g/mL) could effectively inhibit the growth of AGS gastric cancer cells at higher concentrations (P<0.05, P<0.01). The annexin V & dead cell staining assay and cell cycle arrest assay confirmed a dose-dependent increase in the apoptosis rate and G@*CONCLUSION@#ZPE decreases AGS cell proliferation and induces apoptosis by inhibiting Akt and MDM2 expression.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Stomach Neoplasms
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Plant Extracts
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Signal Transduction
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Tumor Suppressor Protein p53
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Apoptosis
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Zanthoxylum
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Cell Line, Tumor
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Cell Proliferation
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Proto-Oncogene Proteins c-akt
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Mice, Inbred BALB C
Limits:
Animals
Language:
En
Journal:
Chinese journal of integrative medicine
Year:
2021
Type:
Article