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Establishment of AML Mouse Model by Transplantation of Hematopoietic Cells from MLL-AF9 Transgenic Mice / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1369-1374, 2021.
Article in Chinese | WPRIM | ID: wpr-922267
ABSTRACT
OBJECTIVE@#To establish a leukemia mouse model induced by transplantation of hematopoietic cells from mixed lineage leukemia (MLL)-AF9 transgenic mice so as to provide the basis for the mechanism research and drug screening of acute myeloid leukemia (AML).@*METHODS@#MLL-AF9 knock-in mice were bred and identified. When the mice developed leukemia, white blood cell (WBC) count in peripheral blood, flow cytometry and morphology method were analyzed to identify the disease. When the WBC count in peripheral blood was more than 100×10@*RESULTS@#The natural onset times of leukemia on MLL-AF9 knock-in mice were 22-28 weeks. The spleens of the transgenic mice enlarged and the bone marrow showed the immature forms of myeloid leukemia cells. Both the bone marrow and spleen cells highly expressed myeloid markers, CD11b and Gr-1. At least 0.5×10@*CONCLUSION@#The leukemia model of hematopoietic cell transplantation based on MLL-AF9 transgenic mice is successfully established, which can be used for the study of the pathogenesis and evaluation of therapeutic effect of AML.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Mice, Transgenic / Leukemia, Myeloid, Acute / Oncogene Proteins, Fusion / Hematopoietic Stem Cell Transplantation / Mice, Inbred C57BL Limits: Animals Language: Chinese Journal: Journal of Experimental Hematology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Mice, Transgenic / Leukemia, Myeloid, Acute / Oncogene Proteins, Fusion / Hematopoietic Stem Cell Transplantation / Mice, Inbred C57BL Limits: Animals Language: Chinese Journal: Journal of Experimental Hematology Year: 2021 Type: Article