Establishment of Cytarabine-resistant Acute Lymphoblastic Leukemia Cell Lines and Its Resistance Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1403-1410, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-922272
ABSTRACT
OBJECTIVE@#To establish cytarabine-resistant acute lymphoblastic leukemia (ALL) cell lines and investigate its possible resistant mechanism.@*METHODS@#Low-concentration cytarabine (Ara-C) continuously induced and cultured Jurkat and Nalm-6 cells to construct cytarabine-resistant cell lines Jurkat/Ara-C and Nalm-6/Ara-C. The cell viability was detected by CCK-8 assay, and the distribution of cell cycle was detected by flow cytometry. Real-time fluorescence quantitative PCR was used to detect the mRNA expression levels of multidrug resistant gene and Ara-C metabolic enzymes. The expression levels of cyclin were detected by Western blot.@*RESULTS@#Jurkat/Ara-C and Nalm-6/Ara-C drug-resistant cell lines were successfully established, the resistance index of which was 1 973.908±161.163 and 7 231.643± 1 190.624, respectively. Drug-resistant cell lines had no cross-resistance to commonly used chemotherapeutic drugs, such as doxorubicin. Flow cytometry showed that the ratio of G@*CONCLUSION@#Cytarabine-resistant ALL cell lines are successfully established by using low concentration continuous induction method, and its drug-resistant mechanism may be related to the deficiencies of DCK and cyclinB1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Line
/
Drug Resistance, Neoplasm
/
Cytarabine
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
ATP Binding Cassette Transporter, Subfamily G, Member 2
/
Neoplasm Proteins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2021
Type:
Article
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