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The Effect of MicroRNA-143 on the Proliferation and Apoptosis of Leukemia Cell Line U-937 / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1695-1703, 2021.
Article in Chinese | WPRIM | ID: wpr-922320
ABSTRACT
OBJECTIVE@#To investigate the expression of microRNA-143 (miR-143) in patients with acute myeloid leukemia (AML), and the effect of miR-143 over-expression on the proliferation and apoptosis of AML cells. And to verify the targeting relationship between miR-143 and long non-coding RNA MALAT-1. So as to explore the possible regulatory mechanism of miR-143 in the pathogenesis of AML.@*METHODS@#The expression of miR-143 in bone marrow cells of AML patients was detected by RT-qPCR. After miR-143 was over-expressed in U-937 cell lines, the proliferation of U-937 cell lines was detected by CCK-8, clone formation assay, and flow cytometry. In addition, cell apoptosis was detected by Hoechst 33258 fluorescence staining and flow cytometry. At the same time, bioinformatics, RT-qPCR and dual luciferase reporter gene assay were used to predicted and verified the targeting relationship between miR-143 and MALAT-1.@*RESULTS@#Expression of miR-143 in AML patients was significantly lower than those in normal controls. Over-expressed miR-143 could inhibit the proliferation of U-937 cells and promote the apoptosis of the cell. The miR-143 binding site was located on the MALAT-1 RNA sequence, and MALAT-1 was down-regulated in U-937 cells after over-expressed miR-143. However, the expression of miR-143 showed no significantly changed after MALAT-1 silencing.@*CONCLUSION@#Expression of miR-143 in AML patients is lower than that in normal controls. Over-expression of miR-143 can inhibit the proliferation of U-937 cells and promote its apoptosis. And its mechanism may be related to its targe regulation of MALAT-1.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Leukemia, Myeloid, Acute / Cell Line / Apoptosis / MicroRNAs / Cell Proliferation Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Leukemia, Myeloid, Acute / Cell Line / Apoptosis / MicroRNAs / Cell Proliferation Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2021 Type: Article