Determination of dexamethasone sodium phosphate in the vitreous by high performance liquid chromatography
Korean Journal of Ophthalmology
;
: 79-83, 1995.
Article
in English
| WPRIM
| ID: wpr-92429
ABSTRACT
Dexamethasone sodium phosphate (DSP) is increasingly used in the treatment of ocular inflammatory diseases by systemic, periocular, and recently, intravitreal injection. We have developed a method for the determination of vitreous levels of DSP by reverse phase HPLC. In this method, co-elution of vitreous proteins with DSP is resolved by a prior sample clean-up procedure using Waters Sep-Pak C18 cartridge; the protein is separated and eluted with water while DSP, paraben and prednisone are eluted with methanol. DSP in the resulting sample is then separated by reverse phase HPLC and quantified by UV absorption at 254 nm. The recovery of DSP through the sample clean-up is 68.9 +/- 3.0%. DSP quantitation is linear from 0.1 mg to 1.0 mg per 1.0 ml vitreous. This method provides a simple, sensitive and reliable technique for determining the vitreous levels of DSP.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vitreous Body
/
Dexamethasone
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Anti-Inflammatory Agents
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Korean Journal of Ophthalmology
Year:
1995
Type:
Article
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