Your browser doesn't support javascript.
loading
Effects of arctiin relieving inflammatory injury of human nasopharyngeal epithelial cells induced by lipopolysac- charide / 中国药房
China Pharmacy ; (12): 1436-1441, 2022.
Article in Chinese | WPRIM | ID: wpr-927189
ABSTRACT
OBJECTIVE To investigate the effect of arctiin (ARC)relieving lipopolysaccharide (LPS)induced inflammatory injury of human nasopharyngeal epithelial cells NP- 69. METHODS The effects of 24 h treatment of 0.000 1,0.001,0.01,0.1, 1.0,10 μmol/L ARC on the proliferation of NP-69 were determined by MTS method. After 0.01,0.1,and 1.0 μmol/L ARC was applied to NP- 69 for 24 h and NP- 69 was pre-treated with 0.01,0.1 and 1.0 μmol/L ARC for 24 h,and then stimulated with 1.0 μg/mL LPS for 24 h,scratch tests were used to detect cell migration in both experiments. LPS stimulated NP- 69 to establish an inflammation injury model. The levels of nitric oxide (NO),tumor necrosis factor α(TNF-α)interleukin-6IL-6),and IL- 1β in cell supernatants were detected ,and mRNA and protein expression of zonula oecludens protein 1(ZO-1),β-defensin 3(BD3), Janus kinase 1 (JAK1),signal transducer and activator of transcription 3 (STAT3) in cell supernatant were also detected. RESULTS Compared with normal group ,0.000 1,0.001,0.01,0.1,1.0,10 μmol/L ARC had no effect on the proliferation of NP-69 after 24 h treatment (P>0.05). ARC (0.1,1.0 μmol/L)could significantly promote the rate of cell migration (P<0.05). For the inflammatory injure of NP- 69 cells stimulated by LPS ,ARC(1.0 μmol/L)could significantly reduce the release of NO , TNF-α and IL-6(P<0.05),significantly increased mRNA and protein expression of ZO- 1 and BD 3 but decreased mRNA and protein expression of STAT 3(P<0.01 or P<0.05). CONCLUSIONS ARC has the effect of reducing the inflammatory injury of NP-69 cells induced by LPS ,promoting the physical and immune defense ability of the nasal mucosa epithelial barrierunder inflammatory environment. The mechanism of action may be related to inhibiting IL- 6/JAK1/STAT3 signaling pathway.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: China Pharmacy Year: 2022 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: China Pharmacy Year: 2022 Type: Article