Application of nanopore sequencing in environmental microbiology research / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 5-13, 2022.
Article
in Chinese
| WPRIM
| ID: wpr-927688
ABSTRACT
The development of high-throughput sequencing techniques enabled a deeper and more comprehensive understanding of environmental microbiology. Specifically, the third-generation sequencing techniques represented by nanopore sequencing have greatly promoted the development of environmental microbiology research due to its advantages such as long sequencing reads, fast sequencing speed, real-time monitoring of sequencing data, and convenient machine carrying, as well as no GC bias and no PCR amplification requirement. This review briefly summarized the technical principle and characteristics of nanopore sequencing, followed by discussing the application of nanopore sequencing techniques in the amplicon sequencing, metagenome sequencing and whole genome sequencing of environmental microorganisms. The advantages and challenges of nanopore sequencing in the application of environmental microbiology research were also analyzed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Environmental Microbiology
/
Metagenome
/
High-Throughput Nucleotide Sequencing
/
Nanopores
/
Nanopore Sequencing
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2022
Type:
Article
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